|
KMID : 0376219810180010101
|
|
Chonnam Medical Journal
1981 Volume.18 No. 1 p.101 ~ p.113
|
|
|
Electron Microscope Autoradiegraphic Study of APUD Cells in Rabbit Intestinal Mucosa.
|
|
|
|
|
Abstract
|
|
|
Electron microscopic autoradiography might be one of the best technique for the study of uptake, synthesis and storage of biogenic amines. This experiment was performed to study the uptake and intracellular distrbution of dopamine after injection of 3H-dopa as a precursor of dopamine by electron microscopic autoradiography.
One hour after intravenous injection of 3H-dopa, the pieces of rabbit duodenum and colon were removed. They were fixed overnight in a 2.5% glutaraldehyde solution in 0.1M cacodylate buffer (pH 7.4) and post-fixed for one hour in 1.5% osmium tetroxide. After dehydration through a graded ethanol series and were embedded in epoxy resion.
Ultrathin sections were affixed on a 2 % celloidin coated glass slide, covered with a monolayer of ILFORD L4 emulsion by dipping method, exposed at 4°C in a light-tight box for two months, developed in microdol X, and fixed in 30% sodium hyposulfide.
Five different types endocrine cells in duodenum and two types in colon were clearly identified by electron microscope. :dost of the endocrine cells in duodenal mucosa except the cells of type 4 and 5, and those in colon had a large number of specific silver grains which represent newly-synthesized dopamine on the irsecretory granules one hour after an intravenous injection of 3H-dopa.
Based upon the above results, it can be inferred that the endocrine cells of the rabbit intestinal mucosa belong to the APUD series.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|